Tuesday, March 26, 2013

Basics Of A Real Time PCR Kit

By Tamra Ferrell


A real time pcr kit is a product that can be used to observe dna material. The reaction is observed in all stages, with dyes or other markers used in the process. The method is reportedly an accurate way to quantify dna. It is being used for research and evaluation purposes in various industries.

It has been useful in the medical community because it can help in diagnosis. When patients come in for treatment, it may not be immediately obvious what has caused their illness. By having a test done using this product, the correct diagnosis can be provided. This is also a tool used to observe new types of illnesses.

The food industry can also benefit from these products. They can be used to observe the bacteria or pathogens are present in a food item. Scientists can use these to observe the various reactions that are happening in the food. It can also be used to ensure that the water is safe enough for public consumption.

It is a useful tool for research, given the range of organisms that are being studied. It takes research to whole new level because a virus, for example, can be assessed on the dna level. Scientists can observe the changes in the organism when the environment changes. They can even determine how the gene is being expressed.

There is another method that relies on the polymerase reaction but there is one major difference. Real time means that the reaction is observed when it is happening. The traditional method on the other hand observes the reaction once it is finished. This limits the results and more processing may be necessary.

You are not sacrificing the sensitivity of your equipment. The results will still be reliable and at par with the results obtained from traditional equipment. The test will be performed in a closed environment so that there is less risk of contaminating the samples inside. Using such equipment is also not hard.

Just as a machine has different models, this product also has different versions. Check what systems are available and familiarize yourself with the capabilities of each system. Determine how accurate the data will be. Ask how much time is needed before one can get an assessment. Moreover, consider what software is to be used and how much everything will cost.

The product can be purchased from different companies. Check the background of your prospects to ensure that they are indeed credible sellers. It will also be advantageous to choose a company that provides support even after the purchase. You will need someone for maintenance or if there are any technical issues.

A real time pcr kit can be very useful for research and diagnostic purposes. This type of product is more specific compared to items that rely on the traditional method because it observes the reaction as it progresses. Different products rely on different systems so it can be tricky to determine which product is suitable. Study the available products first before you make a decision.




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